IAT (IISER) 2023 | Biotechnology: Principles and Processes Question 3 | Biology

A 1000 base-pair DNA fragment was cloned between Hind III and EcoRI sites of the plasmid vector (plAT) of size 3500 base-pair. The cloned fragment had a Not I site as shown in the figure. In order to confirm the presence of the insert, the recombinant plasmid was digested completely with (a) Not I and EcoR I, and (b) Not I and Hind III.

IAT (IISER) 2023 Biology - Biotechnology: Principles and Processes Question 2 English

In lane 1 the products of the digestion by Not I and EcoRI was loaded. In lane 2 the products of the digestion by Not I and Hind III was loaded. Which one of the following correctly represents the agarose gel electrophoresis profile of the digested recombinant plasmid for (a) and (b), respectively?

IAT (IISER) 2023 Biology - Biotechnology: Principles and Processes Question 2 English Option 2

IAT (IISER) 2023 Biology - Biotechnology: Principles and Processes Question 2 English Option 3

IAT (IISER) 2023 Biology - Biotechnology: Principles and Processes Question 2 English Option 4

IAT (IISER) 2022

MCQ (Single Correct Answer)

-1

As opposed to DNA replication within the cell, discontinuous synthesis of DNA does NOT occur in a polymerase chain reaction (PCR). Why?

The replication fork is formed and DNA ligase activity of the Taq polymerase joins the discontinuous fragments.

The replication fork is formed and Taq polymerase extends DNA in both $3^{\prime}$ to $5^{\prime}$ and $5^{\prime}$ to $3^{\prime}$ direction.

Denaturation step in PCR substitutes for the replication fork and Taq polymerase extends DNA only in the $3^{\prime}$ to $5^{\prime}$ direction.

Denaturation step in PCR substitutes for the replication fork and Taq polymerase extends DNA only in the $5^{\prime}$ to $3^{\prime}$ direction.

Questions Asked from MCQ (Single Correct Answer)

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